We require a third-party lab support to assess its effectiveness of a novel food against Cryptosporidium parvum and a selected Streptococcus strain. The project involves spiking a liquid food matrix with C. parvum, initial enumeration, off-site processing, and post-processing enumeration to evaluate reductions in viability and infectivity. While a mouse model is the gold standard for infectivity, a validated cell culture model may suffice. Viability assessment will likely use nuclear staining and flow cytometry. A similar strategy will be applied to the Streptococcus strain.
Due to matrix complexity, method development and a trial run may be needed. The top priority is finding a lab capable of handling Cryptosporidium and performing these analyses.
