Quantifying Acrylamide in Heat-Processed Foods
Project Description
MULTIPLE TESTING
Analytical laboratory (the Philippines preferred) needed to quantify acrylamide in commonly consumed heat-processed and carbohydrate-rich foods using the GC-MS/MS detection method. We'd also like the lab to extract acrylamide from various food samples, following a modified protocol (Jezussek & Schieberle, 2003), which we will implement in our analysis.
Methodology:
1. Sample Collection: We plan to collect commonly consumed heat-processed and carbohydrate-rich foods in the Philippines, which will be vacuum-packed and stored at -80°C until analysis.
2. Chemicals and Reagents: The chemicals involved include acrylamide (50 μg/mL), [13C3]-acrylamide as an internal standard, and other reagents such as acetonitrile/H2O (85:15), n-hexane, ethyl acetate, and sodium sulfate.
3. Acrylamide Extraction: The extraction process includes homogenization with acetonitrile/H2O, sonication, liquid-liquid extraction with n-hexane, and application on an Extrelut NT 20 cartridge for elution with ethyl acetate.
4. Standard Preparation: Calibration standards for quantification are prepared using [13C3]-acrylamide stock solution, and acrylamide stock solution, with concentrations ranging from 0.023 to 2.0 μg/mL.
5. Acrylamide Quantification: Quantification will be done using an Agilent 8890 GC system coupled with an Agilent 7000D triple quadrupole mass spectrometer, employing multiple reaction monitoring for detection.
6. Method Validation: We will validate the method in terms of precision, accuracy, and sensitivity, ensuring that %RSD values remain below 20% and LoD and LoQ are set at 2.10 μg/kg and 7.60 μg/kg, respectively.
We would greatly appreciate it if you could confirm whether your facility can perform the analysis using this method or recommend any alternatives that might suit our needs.
Thank you very much for your time and consideration.
Project Information
Number:24-01816
Industry:Chemicals
